| 蘇秀麗1,3, 李惠敏3, 唐 輝1,2*, 李良波4, 劉寶玉1,2 |
| 1. 廣西壯族自治區 中國科學院 廣西植物研究所, 廣西 桂林 541006;2. 廣西植物功能物質與資源持續利用重點實驗室, 廣西 桂林 541006;3. 廣西師范大學珍稀瀕危動植物生態與環境保護教育部重點實驗室, 廣西 桂林 541006;4. 廣西中醫藥大學 藥學院, 南寧 530200 |
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| 摘要: |
| 為充分開發黃花倒水蓮( Polygala fallax)的內生真菌資源,獲得具有抗植物病原真菌、抗氧化活性的內生真菌,該文以黃花倒水蓮內生真菌為研究對象,使用平板對峙法檢測內生真菌對6種植物病原真菌的抑菌活性,測定內生真菌發酵液的DPPH清除自由基能力和總還原能力,評價內生真菌的抗氧化活性,并對具有強抑菌活性和抗氧化活性的菌株進行形態和ITS鑒定。結果表明:(1)黃花倒水蓮內生真菌中有2株內生真菌對香蕉專化尖孢鐮刀菌、柑橘樹脂病菌、葉點霉菌、香蕉具條葉斑病菌、茄病鐮刀菌、三七根腐病菌具有明顯的抑菌活性,抑菌率在50.3%～91.4%之間,其中HNLF-5對柑橘樹脂病菌的抑菌率為73.2%,HNLF-44對香蕉專化尖孢鐮刀菌抑菌率為91.4%。(2)內生真菌發酵液具有良好的抗氧化活性,DPPH清除率均在80%以上,總還原能力吸光值范圍為0.279 2～0.748 8。(3)HNLF-44菌株為鏈格孢屬真菌。該研究表明,藥用植物黃花倒水蓮內生真菌具有較好的生物活性,為后續從黃花倒水蓮內生真菌中挖掘潛在新型抑菌活性和抗氧化活性物質奠定了基礎。 |
| 關鍵詞: 黃花倒水蓮, 內生真菌, 抗氧化, 抑菌, 菌株鑒定 |
| DOI：10.11931/guihaia.gxzw202112017 |
| 分類號:Q946.885 |
| 文章編號:1000-3142(2023)01-0088-08 |
| 基金項目:廣西科技重大專項(桂科AA18118015); 桂林市重大專項(20190101); 廣西植物功能物質研究與利用重點實驗室項目(ZRJJ2018-7)。 |
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| Bioactivity evaluation of endophytic fungi of Polygala fallax and identification of HNLF-44 strain |
| SU Xiuli1,3, LI Huimin3, TANG Hui1,2*, LI Liangbo4, LIU Baoyu1,2 |
| 1. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;2. Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guilin 541006, Guangxi, China;3. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Guilin 541006, Guangxi, China;4. College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning 530200, China |
| Abstract: |
| The medicinal plant Polygala fallax has a variety of biological activities. In order to fully develop the endophytic fungi resources of the wild P. fallax and to obtain the culture-endophytic fungi strains with an anti-phytopathogenic fungi and antioxidant activities, the endophytic fungi of P. fallax were taken as the research object, the antifungal activity of the endophytic fungi against six kinds of phytopathogenic fungi was detected by the plate confrontation method; and the antioxidant activity of endophytic fungi was evaluated by measuring the DPPH free radical scavenging ability and total reducing ability of endophytic fungi fermentation broth. Finally, the endophytic fungi strains with strong antifungal and antioxidant activities were then analyzed by the morphological traits and the flanking ITS sequencing. The results were as follows:(1)In the antifungal experiment, there were two endophytic fungi of P. fallax had obvious antifungal activities on six kinds phytopathogenic fungi of Fusarium oxysporum f. sp. cubense, Phyllosticta zingiberi, Diaporthe citri, Mycosphaerella fijiensis, Fusarium solani, F. oxysporum, and the inhibition rates ranged from 50.3% to 91.4%. Among them, HNLF-5 strain had a good effect antifungal activity against Diaporthe citri, with a inhibition rate of 73.2%, and HNLF-44 strain had a good effect antifungal activity against Fusarium oxysporum f. sp. cubense, with a inhibition rate of 91.4%.(2)In the experiment of antioxidant activity, the endophytic fungal fermentation broth had good antioxidant activity, the DPPH clearance rate was above 80%, and the total reducing ability absorbance value ranged from 0.279 2 to 0.748 8.(3)It could be seen from the strain identification results that the HNLF-44 strain with antifungal activity was Alternaria sp. fungus. This study indicates that the endophytic fungi of Polygala fallax have a good ability of inhibiting plant phytopathogenic fungi and antioxidant activity, which lays the foundation for the subsequent exploration of potential new antifungal and antioxidant substances from P. fallax endophytic fungi. |
| Key words: Polygala fallax, endophytic fungi, antioxidant, antifungi, identification |
