廣西地不容非藥用部位化學成分研究

張汝勝^1,2, 李霞¹, 黃永林², 王亞鳳², 陽丙媛², 李典鵬², 何瑞杰^2*

1. 桂林理工大學化學與生物工程學院, 廣西桂林 541006;2. 廣西植物功能物質與資源持續利用重點實驗室, 廣西壯族自治區中國科學院廣西植物研究所, 廣西桂林 541006

摘要:

廣西地不容(Stephania kwangsiensis)為防己科藤本植物,主要分布于廣西,其藥用部位(塊根)中藥名為“金不換”,有鎮靜、殺蟲、抗炎、抗腫瘤、抗病毒、抑菌的作用,用于治療阿爾茨海默病。為了充分利用該植物資源,明確廣西地不容非藥用部位中的化學成分,并發現其相關的活性成分。該研究綜合運用正相Sephadex LH-20、正相硅膠、正相聚酰胺、MCI gel CHP-20P等多種柱色譜法對廣西地不容非藥用部位甲醇提取物進行分離純化,所得單體化合物的結構經MS、¹H-NMR、¹³C-NMR等波譜學數據分析及相關文獻對照進行鑒定,同時用濾紙法對分離得到的化合物開展抗細菌(大腸桿菌、金色葡萄球菌、枯草芽孢桿菌)和抗真菌(尖孢鐮刀菌)的活性評價。結果表明:(1)從該植物中分離得到的10個化合物分別鑒定為吐葉醇(1)、蚱蜢酮(2)、異紫堇定堿(3)、2-O-β-D-glucopyranosyl-D-arabinitol(4)、(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5)、magnoflorine(6)、corchoionoside C(7)、委陵菜酸(8)、紫堇定堿(9)、克班寧(10)。其中,化合物1、2、4、5、6、7、8為首次從該植物中分離得到,化合物1、2、4、5、7、8為首次從該屬植物中分離得到,化合物4為首次從天然產物中分離得到。(2)在化合物濃度為6 mg·mL^-1時,化合物1、2、5對大腸桿菌具有弱抑菌作用,化合物2對金色葡萄球菌有弱的抑菌活性,化合物3和化合物8對枯草芽孢桿菌有弱的抑制作用。綜上表明,廣西地不容非藥用部位含有豐富的活性成分,具有開發利用的潛力。

關鍵詞: 廣西地不容, 化學成分, 結構鑒定, 生物堿, 抑菌活性

DOI：10.11931/guihaia.gxzw202108008

分類號:Q946.88

文章編號:1000-3142(2023)01-0103-08

基金項目:國家自然科學基金(32060108,82060764); 廣西自然科學基金(2018GXNSFAA294033,2018GXNSFAA281078); 中國博士后專項基金(200626); 廣西植物研究所基本業務費項目(桂植業18004); 廣西創新驅動發展專項基金(桂科AA18118015); 廣西植物功能物質與資源持續利用重點實驗室開放基金(ZRJJ2020-2,ZRJJ2018-19); 省部共建藥用資源化學與藥物分子工程國家重點實驗室開放基金(CMEMR2019-B13); 廣東省重點領域研發計劃項目(2020B1111110003); 中央引導地方科技發展專項(桂科20111010)。

Chemical constituents from non-medicinal parts of Stephania kwangsiensis

ZHANG Rusheng^1,2, LI Xia¹, HUANG Yonglin², WANG Yafeng², YANG Bingyuan², LI Dianpeng², HE Ruijie^2*

1. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, Guangxi, China;2. Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China

Abstract:

Stephania kwangsiensis, a vine plant of the Stephaniaceae family, is mainly spread in Guangxi, China. The medicinal part(root tuber)of S. kwangsiensis, “Jin Bu Huan” in Chinese, is often used for sedative, insecticidal, anti inflammatory, anti-tumor, antiviral and antibacterial, as well as for the treatment of Alzheimer's disease. In order to make full use of the plant resources, clarify its chemical constituents, and find the relevant active compounds, the methanol extracts of non-medicinal parts of this plant were isolated using various chromatographic methods such as positive phase Sephadex LH-20, positive phase silica gel, positive phase polyamide, and MCI gel CHP-20P. The structures of the isolated monomer compounds were determined by MS,¹H-NMR, ¹³C-NMR, and other spectral data analysis, as well as comparison with relevant literatures. The activities of the isolated compounds from S. kwangsiensis against bacteria(Escherichia coli, Staphylococcus aureus, Bacillus subtilis)and fungi(Fusarium oxysporum)were evaluated using filter paper method. The results were as follows:(1)A total of 10 compounds obtained from the non-medicinal parts of this plant were identified as vomifoliol(1), grasshopper ketone(2), isocorydine(3),2-O-β-D-glucopyranosyl-D-arabinitol(4),(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5), magnoflorine(6), corchoionoside C(7), tormentic acid(8), corydine(9)and crebanine(10). Among them, compounds 1, 2, 4, 5, 6, 7 and 8 were isolated from this plant for the first time, compounds 1, 2, 4, 5, 7 and 8 were firstly obtained from this genus plants, and Compound 4 was found from natural products for the first time.(2)At concentration of 6 mg·mL^-1, compounds 1, 2 and 5 showed weak antibacterial activities against Escherichia coli; Compound 2 exhibited weak antibacterial activities against Staphylococcus aureus; compounds 3 and 8 displayed weak antibacterial activities against Bacillus subtilis. In conclusion, the non-medicinal parts of Stephania kwangsiensis contain large numbers of active components and have the potential of development and utilization.

Key words: Stephania kwangsiensis, chemical constituentes, structural identification, alkaloid, antibacterial activity