| 朱成豪1, 鄒 蓉2, 唐健民2, 韋 霄2, 孫志蓉1, 史艷財2* |
| 1. 北京中醫藥大學 中藥學院, 北京 102488;2. 廣西壯族自治區 中國科學院 廣西植物研究所, 廣西 桂林 541006 |
|
| 摘要: |
| 為深入認識特色植物莖花山柚(Champereia manillana var. longistaminea)的藥效物質基礎,該研究開展了莖花山柚葉95%乙醇提取物的化學成分研究。結果表明:(1)采用硅膠柱層析(CC)、薄層色譜(TLC)、葡聚糖凝膠柱層析(Sephadex LH-20)、反相硅膠柱層析(RPC18)與高效液相色譜(HPLC)等方法,對莖花山柚葉乙醇浸提物的乙酸乙酯萃取部位進行了分離純化,得到6個單體化合物。(2)通過核磁共振波譜(NMR)和高分辨質譜(HR-ESI-MS)數據,并結合文獻數據對比鑒定了這些化合物的結構,6個化合物分別為蒲公英賽醇(1)、吲哚-3-甲酸(2)、(24R)-環菠蘿蜜烷-3β,24,25-三萜(3)、(24R,S)-3β-24,31-環氧-24-甲基環烷(4)、1-O-亞麻酰基-3-O-β-D-吡喃半乳糖基-sn-丙三醇(5)、長烷基鏈甘油單酯(6)。其中,化合物1-6均為首次從該植物中分離得到。 |
| 關鍵詞: 莖花山柚, 提取分離, 純化, 化學成分, 結構鑒定 |
| DOI：10.11931/guihaia.gxzw202111048 |
| 分類號:Q946 |
| 文章編號:1000-3142(2023)01-0120-06 |
| 基金項目:廣西科技基地和人才專項(桂科計字 [2021]220號); 廣西植物研究所基本科研業務費項目(桂植業21002)。 |
| |
| Chemical constituents from the leaves of Champereia manillana var. longistaminea |
| ZHU Chenghao1, ZOU Rong2, TANG Jianmin2, WEI Xiao2, SUN Zhirong1, SHI Yancai2* |
| 1. School of Chinese Materia, Beijing University of Chinese Medicine, Beijing 102488, China;2. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China |
| Abstract: |
| In order to study the chemical constituents in the leaves of Champereia manillana var. longistaminea, the chemical composition was analyzed by the silica gel column chromatography(CC), thin-layer chromatography(TLC), dextran gel column chromatography(Sephadex LH-20), reversed-phase silica gel(RPC18)column chromatography and high performance liquid chromatography(HPLC). The ethyl acetate extraction part of the ethanol extraction was separated and six monomer compounds were obtained. The structures of these compounds were identified using nuclear magnetic resonance spectroscopy(NMR), high resolution mass spectrometry(HR-ESI-MS)and the data of the literature. Six compounds were elucidated as taraxerol(1), 1H-indole-3-carboxylic acid(2),(24R)-cycloartane-3β, 24, 25-triol(3),(24R, S)-3β-24,31-epoxy-24-methylcycloartane(4), 1-O-linolenoyl-3-O-β-D-galactospyranosyl-snglycerol(5), and hyloglyceride(6). All compounds 1-6 were isolated from this plant for the first time. |
| Key words: Champereia manillana var. longistaminea, extraction and separation, purification, chemical constituents, structure identification |
莖花山柚葉的化學成分研究.pdf
