| 盛天露1, 付璐鷺1, 許俊申1, 尚銳峰1, 陳鐘文1, 劉 峰2, 劉 華1* |
| 1. 江西中醫(yī)藥大學(xué) 藥學(xué)院, 南昌 330004;2. 江西中醫(yī)藥大學(xué)附屬醫(yī)院, 南昌 330006 |
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| 摘要: |
| 蒼耳(Xanthium strumarium)為民間傳統(tǒng)藥用植物,本課題組前期研究發(fā)現(xiàn)蒙古蒼耳大孔樹脂50%乙醇洗脫部位具有顯著的抑制細(xì)胞壞死性凋亡(necroptosis)活性。為明確蒙古蒼耳(X. mongolicum)的活性成分,活性部位采用反復(fù)硅膠柱色譜、Sephadex LH-20柱色譜、制備高效液相色譜、重結(jié)晶等多種方法進(jìn)行分離和純化,運(yùn)用NMR、MS等波譜方法并結(jié)合文獻(xiàn)數(shù)據(jù)對分離得到的化合物進(jìn)行結(jié)構(gòu)鑒定。結(jié)果表明:從該活性部位中分離得到14個化合物,分別鑒定為hydroxydihydrobovolide(1)、樹莓酮(2)、水楊醇(3)、對羥基苯乙酮(4)、對羥基苯甲醛(5)、咖啡酸乙酯(6)、阿魏醛(7)、異東莨菪素(8)、3,3'-bis(3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran)(9)、axillarin(10)、槲皮素(11)、(+)松脂素(12)、β-谷甾醇(13)和棕櫚酸(14)。化合物1-4、7-10均為首次從蒼耳中分離得到。 |
| 關(guān)鍵詞: 蒼耳, 壞死性凋亡, 活性部位, 化學(xué)成分, 江西 |
| DOI：10.11931/guihaia.gxzw202108047 |
| 分類號:Q946 |
| 文章編號:1000-3142(2023)01-0132-07 |
| 基金項(xiàng)目:國家自然科學(xué)基金(81760706); 江西省中醫(yī)藥大學(xué)中藥學(xué)一流學(xué)科專項(xiàng)科研基金(JXSYLXK-ZHYAO022,JXSYLXK-ZHYAO112)。 |
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| Chemical constituents of the necroptosis-inhibited active fraction from Xanthium mongolicum(Ⅰ) |
| SHENG Tianlu1, FU Lulu1, XU Junshen1, SHANG Ruifeng1, CHEN Zhongwen1, LIU Feng2, LIU Hua1* |
| 1. College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China;2. Affiliated Hospital, Jiangxi University of Chinese Medicine, Nanchang 330006, China |
| Abstract: |
| Xanthium strumarium is a traditional folk medicinal plant. The 50% ethanol eluting faction of X. mongolicum had significant activity to inhibit necroptosis. In order to investigate the active constituents, the active fraction of X. mongolicum were isolated and purified by various chromatographic methods such as repeated silica gel column chromatography, Sephadex LH-20 column chromatography, preparative high performance liquid chromatography and recrystallization. Their structures were established on the basis of NMR, MS spectraoscopic analyses and comparison with lirerature date. The results were as follows: A total of 14 compounds were isolated from the active part and identified as hydroxydihydrobovolide(1), raspberry ketone(2), salicyl alcohol(3), 4-hydroxyl-acetophenone(4), 4-hydroxybenzaldehyde(5), ethyl caffeate(6), ferulaldehyde(7), isoscopletin(8), 3,3'-bis(3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran)(9), axillarin(10), quercetin(11),(+)pinoresinol(12), β-sitosterol(13)and palmatic acid(14). Compounds 1-4, 7-10 were all isolated from Xanthium mongolicum for the first time. |
| Key words: Xanthium mongolicum, necroptosis, active fraction, chemical constituents, Jiangxi |
蒙古蒼耳抑制壞死性凋亡(ｎｅｃｒｏｐｔｏｓｉｓ)__活性部位化學(xué)成分研究(Ⅰ).pdf
