| 宋靜靜1, 羅美苗1, 李 露1, 楊子聰1, 王琳1, 諸葛才智1, 邱清華2, 朱開昕3, 蘇本偉3, 龔 斌1* |
| 1. 廣西北部灣海洋生物多樣性養(yǎng)護(hù)重點(diǎn)實(shí)驗(yàn)室, 北部灣大學(xué) 海洋學(xué)院, 廣西 欽州535011;2. 北部灣大學(xué) 陶瓷與設(shè)計(jì)學(xué)院, 廣西 欽州535011;3. 欽州市中醫(yī)醫(yī)院, 廣西 欽州535099 |
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| 摘要: |
| 為研究夾竹桃(Nerium oleander)內(nèi)生真菌的多樣性并評價其次生代謝產(chǎn)物的活性,該研究對廣西夾竹桃(Nerium oleander)的內(nèi)生真菌進(jìn)行分離純化,采用形態(tài)學(xué)和ITS序列分析結(jié)合的方法進(jìn)行鑒定,以5種指示菌(其中有3種弧菌)對內(nèi)生真菌提取物進(jìn)行抑菌活性篩選。結(jié)果表明:(1)從廣西夾竹桃中共得到19株內(nèi)生真菌,這19株內(nèi)生真菌都屬于子囊菌門,涵蓋5個目7個屬,包括炭疽菌屬(Colletotrichum)、球座菌屬(Guignardia)、葉點(diǎn)霉屬(Phyllosticta)、新殼梭孢屬(Neofusicoccum)、曲霉屬(Aspergillus)、隔孢殼科新屬(Nothophoma)和間座殼屬(Diaporthe),優(yōu)勢屬為炭疽菌屬(分離率為36.85%)和球座菌屬(分離率為21.05%),其中炭疽菌屬主要分布于莖,球座菌屬全部來源于葉。(2)jing-117(Neofusicoccum sp.)和ye-130(Guignardia sp.)對坎氏弧菌有較為特異的抑菌效果,ye-136(Aspergillus sp.)能同時抑制蠟樣芽孢桿菌和坎氏弧菌,ye-135(Aspergillus sp.)和jing-116(Colletotrichum sp.)僅能抑制蠟樣芽孢桿菌,ye-134(Guignardia sp.)對溶藻弧菌有抑制作用。該研究首次基于ITS序列揭示了廣西夾竹桃內(nèi)生真菌存在較為豐富的多樣性,并篩選到一些有抑菌活性的菌株,其提取物可以抑制水產(chǎn)病原弧菌的生長,具有較好的開發(fā)潛力。 |
| 關(guān)鍵詞: 夾竹桃, 內(nèi)生真菌, ITS序列分析, 弧菌, 抑菌活性 |
| DOI：10.11931/guihaia.gxzw202105037 |
| 分類號:Q945.8 |
| 文章編號:1000-3142(2023)01-0148-07 |
| 基金項(xiàng)目:國家自然科學(xué)基金(31560727); 廣西自然科學(xué)基金(2018JJA130187, 2020GXNSFDA238015); 廣西北部灣海洋生物多樣性養(yǎng)護(hù)重點(diǎn)實(shí)驗(yàn)室(北部灣大學(xué))自主基金(2021ZA01, 2019ZC01)。 |
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| Diversity of endophytic fungi isolated from Nerium oleander in Guangxi and activity screening of inhibiting several aquatic pathogens |
| SONG Jingjing1, LUO Meimiao1, LI Lu1, YANG Zicong1, WANG Lin1, ZHUGE Caizhi1, QIU Qinghua2, ZHU Kaixin3, SU Benwei3, GONG Bin1* |
| 1. Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, |
| Abstract: |
| Nerium oleander is an important medicinal plant. In order to study the diversity of endophytic fungi from N. oleander and evaluate the activity of its secondary metabolites, the endophytic fungi of N. oleander in Guangxi were isolated and purified, and identified by a combination of morphology and ITS sequence analysis. Then the antibacterial activities of endophytic fungus extracts were screened with five indicator bacteria(including three Vibrio species). The results were as follows:(1)A total of 19 endophytic fungi were obtained from Guangxi N. oleander. The ITS sequence analysis showed that these 19 endophytic fungi all belonged to the Ascomycota, and covered five orders and seven genera, which including Colletotrichum, Guignardia, Phyllosticta, Neofusicoccum, Aspergillus, Nothophoma and Diaporthe. Among them, the dominant genera were Colletotrichum(the separation rate was 36.85%)and Guignardia(the isolation rate was 21.05%). Colletotrichum was mainly distributed on stems, and Guignardia all originated from leaves.(2)Antibacterial experiments showed that jing-117(Neofusicoccum sp.)and ye-130(Guignardia sp.)had specific antibacterial effects on Vibrio campbellii, ye-136(Aspergillus sp.)could simultaneously inhibit Bacillus cereus and Vibrio campbellii. ye-135(Aspergillus sp.)and jing-116(Colletotrichum sp.)could only inhibit B. cereus, and ye-134(Guignardia sp.)had an inhibitory activity to Vibrio alginolyticus. The study revealed for the first time that endophytic fungi of N. oleander in Guangxi has a relatively rich diversity based on the ITS sequence. Some antibacterial active strains could be screened, and their extracts could inhibit the growth of aquatic pathogen Vibrio, which will has good development value in the future. |
| Key words: Nerium oleander, endophytic fungi, analysis of ITS sequence, Vibrio, antibacterial activities |
廣西夾竹桃內(nèi)生真菌的多樣性和抑制__幾種水產(chǎn)病原菌活性篩選.pdf
