| 黔產(chǎn)大青化學(xué)成分及其抗菌活性研究 |
| 霍曉敏1,2, 李麗君1, 冉堅(jiān)強(qiáng)1, 薛 站1,2, 劉麗娜3, 譚承建1* |
| 1. 貴州民族大學(xué) 民族醫(yī)藥學(xué)院, 貴陽(yáng) 550025;2. 貴州民族大學(xué) 化學(xué)工程學(xué)院, 貴陽(yáng) 550025;3. 貴州醫(yī)科大學(xué) 生物與工程學(xué)院, 貴陽(yáng) 550025 |
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| 摘要: |
| 大青(Clerodendrum cyrtophyllum)是苗族地區(qū)常用的藥用植物。為研究黔產(chǎn)大青的化學(xué)成分及其抗菌活性,該研究采用大孔吸附樹(shù)脂、反相柱色譜、硅膠柱色譜、Sephadex LH-20 柱色譜、半制備高效液相色譜等方法對(duì)大青95%乙醇提取物進(jìn)行分離和純化,并根據(jù)波譜數(shù)據(jù)鑒定所得化合物結(jié)構(gòu),采用MTT法評(píng)價(jià)其抗菌活性。結(jié)果表明:從大青中分離得到18個(gè)化合物,分別鑒定為木栓酮(1)、α-amyrin palmitate(2)、sambuculin A(3)、22-dehydroclerosterol(4)、isopetasin(5)、taraxasteryl palmitate(6)、豆甾醇(7)、22-dehydroclerosterol-3-O-β-D-(6'-O-margaroyl)-glucopyranoside(8)、3-O-β-D-galactopyranosyl-(24β)-ethylcholesta-5,22,25-trien(9)、肉蓯蓉苷D(10)、tortoside F(11)、balanophonin(12)、木犀草苷(13)、類(lèi)葉升麻苷(14)、木犀草素(15)、acantrifoside E(16)、trichotomside B(17)、肉蓯蓉苷C(18),其中化合物2、3、5、6、8-13、16-18均為首次從大青中分離得到。活性測(cè)試結(jié)果表明,化合物13對(duì)乳房鏈球菌有一定的抑制作用。該研究豐富了大青化學(xué)成分,為其在抗奶牛乳房炎藥物的研發(fā)提供了參考。 |
| 關(guān)鍵詞: 大青, 化學(xué)成分, 分離, 鑒定, 抗菌活性 |
| DOI：10.11931/guihaia.gxzw202109081 |
| 分類(lèi)號(hào):Q946 |
| 文章編號(hào):1000-3142(2023)01-0155-10 |
| 基金項(xiàng)目:國(guó)家自然科學(xué)基金(31360084, 31660103, 32160110); 貴州省科技計(jì)劃項(xiàng)目(黔科合支撐 [2019]2414)。 |
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| Chemical constituents and antibacterial activities of Clerodendrum cyrtophyllum in Guizhou |
| HUO Xiaomin1,2, LI Lijun1, RAN Jianqiang1, XUE Zhan1,2, LIU Lina3, TAN Chengjian1* |
| 1.Qinzhou 535011, China;2. College of Ceramies and Design, Beibu Gulf University, Qinzhou 535011, China;3. Qinzhou |
| Abstract: |
| Clerodendrum cyrtophyllum is a common medicinal plant used by Miao people. In order to study the chemical constituents and antibacterial activities of C. cyrtophyllum in Guizhou, China. Macroporous adsorption resin, reversed phase column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatagraphy, and semi-preparative high performance liquid chromatography were used to separate and purify 95% ethanol extracts from C. Cyrtophyllum. The antibacterial activities were evaluated by MTT method. The results showed 18 compounds were isolated and identified as friedelin(1), α-amyrin palmitate(2), sambuculin A(3), 22-dehydroclerosterol(4), isopetasin(5), taraxasteryl palmitate(6), stigmasterol(7), 22-dehydroclerosterol-3-O-β-D-(6'-O-margaroyl)-glucopyranoside(8), 3-O-β-D-galactopyranosyl-(24β)-ethylcholesta-5,22,25-trien(9), cistanoside D(10), tortoside F(11), balanophonin(12), luteoloside(13), acteoside(14), luteolin(15), acantrifoside E(16), trichotomside B(17), cistanoside C(18). Among of them, compounds 2, 3, 5, 6, 8-13, 16-18 were all isolated from C. cyrtophyllum for the first time. Besides,biological activity test showed Compound 13 had antibacterial activity against Streptococcus uberis. The study enriches the chemical composition of C. cyrtophyllum, and provides reference for the drug development of anti-mastitis of dairy cows. |
| Key words: Clerodendrum cyrtophyllum, chemical constituents, isolation, identification, antibacterial activities |
黔產(chǎn)大青化學(xué)成分及其抗菌活性研究.pdf
