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| 賈 凱, 劉 俊, 耿曉桐, 張耀洲, 肖 穎* |
| 信陽(yáng)農(nóng)林學(xué)院 制藥工程學(xué)院, 河南 信陽(yáng)464000 |
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| 摘要: |
| 為確定細(xì)葉十大功勞(Mahonia fortunei)葉中總生物堿大孔樹(shù)脂分離純化的最佳工藝條件及抗氧化活性,該研究通過(guò)比較6種大孔吸附樹(shù)脂對(duì)總生物堿的靜態(tài)吸附和解吸附效果,優(yōu)選出最佳樹(shù)脂并考察其動(dòng)態(tài)純化總生物堿的工藝條件,并采用DPPH法對(duì)純化前后的總生物堿抗氧化性能進(jìn)行評(píng)價(jià)。結(jié)果表明:(1)AB-8型大孔吸附樹(shù)脂純化效果最好,其最佳工藝條件為上樣濃度50 mg·mL-1(生藥濃度)、上樣量26 BV、上樣液流速2 BV·h-1; 吸附完成后,以3 BV水洗后再以4 BV 50%乙醇洗脫,在此條件下得到的總生物堿含量由13.33%提高到56.64%。(2)各樣品對(duì)DPPH自由基的清除能力為對(duì)照品Vc(IC50=10.39 μg·mL-1)>總生物堿純化品(IC50=39.08 μg·mL-1)>總生物堿粗品(IC50=55.28 μg·mL-1)。綜上表明,AB-8型大孔吸附樹(shù)脂可有效富集細(xì)葉十大功勞葉中總生物堿有效部位,細(xì)葉十大功勞葉中總生物堿具有一定的抗氧化活性。 |
| 關(guān)鍵詞: 細(xì)葉十大功勞葉, 總生物堿, AB-8樹(shù)脂, 純化, 抗氧化 |
| DOI：10.11931/guihaia.gxzw202101071 |
| 分類(lèi)號(hào):Q946.88 |
| 文章編號(hào):1000-3142(2023)01-0183-07 |
| 基金項(xiàng)目:河南省重大科技專(zhuān)項(xiàng)(創(chuàng)新示范專(zhuān)項(xiàng))(201111310900); 校級(jí)科技創(chuàng)新團(tuán)隊(duì)建設(shè)項(xiàng)目(CXTD-201805)。 |
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| Purification and antioxidant activity of total alkaloids from leaves of Mahonia fortunei by macroporous resin |
| JIA Kai, LIU Jun, GENG Xiaotong, ZHANG Yaozhou, XIAO Ying* |
| College of Pharmaceutical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, Henan, China |
| Abstract: |
| In order to determine the optimum conditions and antioxidant activity of total alkaloids from leaves Mahonia fortunei by macroporous resins, the best resins were selected by comparing the static adsorption and desorption effects of six kinds of macroporous adsorption resins on total alkaloids. The optimum process conditions for the dynamic purification of total alkaloids were investigated, and the anti-oxidation performance of total alkaloids before and after purification was evaluated by DPPH method. The results were as follows:(1)AB-8 macroporous adsorption resins had the best purification effects. The optimum process conditions were crude herbal dose of 50 mg·mL-1, sample volume of 26 BV, sample flow rate of 2 BV·h-1, eluted successively with 3 BV water and 4 BV 50% ethanol. Under these optimized conditions, the content of total alkaloids was increased from 13.33% to 56.64%.(2)The total alkaloids had good scavenging effects on DPPH· before and after purification. The scavenging rate of DPPH· was Vc(IC50=10.39 μg·mL-1)> purified total alkaloids(IC50=39.08 μg·mL-1)> crude total alkaloids(IC50=55.28 μg·mL-1). AB-8 macroporous adsorption resin can effectively enrich the effective parts of total alkaloids from leaves of Mahonia fortunei, and the total alkaloids have certain antioxidant activities. |
| Key words: Mahonia fortunei leaf, total alkaloids, AB-8 macroporous resin, purification, antioxidation |
細(xì)葉十大功勞葉中總生物堿大孔樹(shù)脂純化及抗氧化研究.pdf
