椿根皮的化學成分及抑菌活性研究

袁亞敏, 周曉歡^*, 王鳳霞, 明虎斌, 何佩娜, 王繼紅

平頂山學院醫(yī)學院, 河南平頂山 467000

摘要:

為探尋椿根皮抑菌的物質(zhì)基礎,該研究采用硅膠、Sephadex LH-20等方法對椿根皮甲醇提取物進行分離和純化,通過理化性質(zhì)和波譜數(shù)據(jù)分析單體化合物的結構,并以卡那霉素為對照組采用流式細胞法測試化合物的抑菌活性。結果表明:從椿根皮中得到22個化合物,分別鑒定為pleuchiol(1)、withastramonolide(2)、7-ketositosterol(3)、白樺酯醇(4)、樺木酸甲酯(5)、1, 2, 4-trimethoxybenzene(6)、順丁烯二酸二甲酯(7)、sonderianol(8)、dibutyl phthalate(9)、pinoresinol(10)、對羥基苯甲酸乙酯(11)、avenalumic acid methyl ester(12)、5,3'-dihydroxy-3,7,4'-trimethoxy-flavone(13)、spathulenol(14)、2-甲基-5-丙基酮-7-羥基色原酮(15)、 7,4'-dihydroxyflavone(16)、annphenone(17)、3-羥基-4-甲氧基苯甲酸(18)、5,3',4'-三羥基-7-甲氧基二氫黃酮(19)、鄰苯二甲酸二丁酯(20)、4-O-甲基沒食子酸(21)、對苯二甲酸二辛酯(22)。所有化合物均為首次從椿根皮中分離得到。抑菌活性測試結果顯示,化合物2對綠膿桿菌、枯草芽孢桿菌均有抑制作用,化合物3對枯草芽孢桿菌有抑制作用,化合物8對綠膿桿菌、金黃色葡萄球菌、枯草芽孢桿菌均有抑制作用,化合物17對綠膿桿菌、金黃色葡萄球菌均有抑制作用。其中,化合物2對枯草芽孢桿菌的抑制作用與卡那霉素無顯著差異(P>0.05)。

關鍵詞: 椿根, 化學成分, 分離純化, 結構鑒定, 抑菌活性

DOI：10.11931/guihaia.gxzw202112078

分類號:Q946

文章編號:1000-3142(2023)01-0190-09

基金項目:河南省高等學校重點科研項目(19A350007); 河南省科技廳科技攻關項目(202102310478)。

Chemical constituents from the root bark of Ailanthus altissima and their antibacterial activities

YUAN Yamin, ZHOU Xiaohuan^*, WANG Fengxia, MING Hubin, HE Peina, WANG Jihong

College of Medical, Pingdingshan University, Pingdingshan 467000, Henan, China

Abstract:

In order to explore the antibacterial material basis from the root bark of Ailanthus altissima, the silica gel and Sephadex LH-20 were employed to separate and purify methanol extract from the root bark of the A. altissima, and the structures of the compounds were identified by chemical properties and spectral data. Flow cytometry was employed to test the antibacterial activity of the compounds, and kanamycin was used as control group. The results were as follows: Twenty-two compounds were isolated and elucidated from the root bark of A. altissima respectively as pleuchiol(1), withastramonolide(2), 7-ketositosterol(3), betulin(4), betulinic acid methyl ester(5), 1, 2, 4-trimethoxybenzene(6), dimethyl maleate(7), sonderianol(8), dibutyl phthalate(9), pinoresinol(10), p-hydroxybenzoic acid ethyl ester(11), avenalumic acid methyl ester(12), 5, 3'-dihydroxy-3,7, 4'-trimethoxyflavone(13), spathulenol(14), 2-methyl-5-acetonyl-7-hydroxychromone(15), 7, 4'-dihydroxyflavone(16), annphenone(17), 3-hydroxy-4-methoxybenzoic acid(18), 5, 3',4'-trihydroxy-7-methoxyflavanone(19), dibutyl phthalate(20), 4-O-methylgallic acid(21), dioctyl terephthalate(22). All compounds were isolated from the root bark of A. altissima for the first time. The antibacterial activity tests showed that Compound 2 had inhibitory effect on Pseudomonas aeruginosa and Bacillus subtilis. Compound 3 had inhibitory effects on Bacillus subtilis. Compound 8 had inhibitory effects on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Compound 17 had inhibitory effects on Pseudomonas aeruginosa and Staphylococcus aureus. The inhibitory effect of Compound 2 on Bacillus subtilis was not significantly different from kanamycin(P>0.05). This paper aims to clarify the antibacterial substance basis of the root bark of A. altissima, and provide a certain theoretical basis for the development and utilization of the root of A. altissima resources and the research and development of drugs with antibacterial activity.

Key words: Ailanthus altissima, chemical constituents, separation and purification, structure identification, antibacterial activity